The fifth edition includes the Aberdeen knot, updated information on suture materials, sterilization techniques, and instrument handling. For the left-handed student, illustrations regarding tying of the Aberdeen knot, and proper instrument grip and release have been added. The techniques of knot tying continue to be the primary focus of the handbook. This handbook will assist those studying to become a veterinarian, physician, nurse practitioner, physician’s assistant, emergency medical technician, midwife, podiatrist, research specialist or food animal producer. Equally important, the book has information for the outdoor enthusiasts or those simply wanting to learn the skills of surgical knot tying. Information about cleaning, sterilization and preparing a surgical field for closing acute wounds and incisions is included.